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Pipette Tips

Pipette Tips

Pipette is a metering tool that removes liquid from the original container to another container within a certain range. Pipettes are widely used in clinical laboratories because of their simple structure and they are very easy to use. So, they are essential instruments in biological research, and the number of pipette tipss used in experiments is enormous. pipette tips is used with pipette and pipettor to speed up processing and reduce cross-contamination. Pipettes and tips are widely used in the field of biology, chemistry, and so on.
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Product Introduction

Company Profile

 

 

Qingdao Root Industrial and Trading Co., Ltd. Was founded in 2008 year, we are committed to provide one-stop custom plastic solution from product design, mold manufacturing, precision machining service and mass production, our molds and products are widely used in Automotive, home appliances, electronics, medical and other industries. We provide secondary processing such as PVD chrome, painting, printing, hot stamping, ultrasonic welding and assembly of plastic parts. Rootsino have our own affiliated factories, occupies an area of more than 3000 square meters; and passed ISO9001 and TS16949 certification, The design team engages in UG, CAD, CAE and CAM design and formulation using computer software programs. They use CAD programs to generate 2D blueprints, UG to made 3D designs, use CAE simulation to identify the optimal fabrication pattern, and then generate CAM computer simulation.

 

 
Why Choose Us
 
01/

Professional Team
Now we have more than 80 employees, 20 of them are experienced engineers in CAD, CAE, CAM skills. Injection department have 28 people.

02/

Advanced Equipment
The existing CNC machine tools, coordinate detection system and various kinds of machine tools are more than 20 units, more than 2000 square meters factory mold.

03/

Company Certificate
In order to better development, our company passed ISO9001 and TS16949 certification.

04/

Rich Experience
With the fast feedback, improved design service, most secure on customer's design, most energy-efficient solution offer, reasonable cost, free mold maintenance when parts injection at our side.

 

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Pipette Tips

Pipette tips are small, disposable plastic tips that are used in conjunction with pipettes for the precise dispensing of liquids. The pipette tips come in different sizes, from microliters to milliliters, and can be used with a variety of pipettes, including single-channel, multi-channel and robotic pipettes

 

What is Pipette Tips

 

 

Pipette is a metering tool that removes liquid from the original container to another container within a certain range. Pipettes are widely used in clinical laboratories because of their simple structure and they are very easy to use. So, they are essential instruments in biological research, and the number of pipette tipss used in experiments is enormous. pipette tips is used with pipette and pipettor to speed up processing and reduce cross-contamination. Pipettes and tips are widely used in the field of biology, chemistry, and so on.
Pipettes are traditionally equipped with a pipette tips at their lower end. The tip is an important part of the pipette. It is a removable unit designed to collect the sample taken. It can be used for any molecular biology and genetics research application. The molecular bioproducts tips form an effective protective structure between the pipette and the sample, ensuring safe aspiration and dispensing.

 

Advantages of Pipette Tips
1

Improved Accuracy

Pipette tipss are designed to provide greater accuracy and precision in liquid handling, ensuring that the exact volume of sample is transferred each time.

2

Decreased Contamination

Pipette tipss serve as a barrier against contamination from one sample to another, preventing any contamination of samples.

3

Consistency and Reproducibility

The use of high-quality pipette tipss ensures the same performance and results every time, ensuring consistency and reproducibility.

4

Ease of Use

Pipette tipss are easy to use, especially when automated systems are used, as they allow for rapid and accurate transfer of liquids.

 

Types of Pipette Tip
 
1. Filter Tips

The generation of aerosols inside the pipette tip upon aspiration of the liquid is quite common. This means that the pipette will be contaminated if filter tips are not used to secure the sample. If you fail to apply a filter tip or end up changing the tips in between, not only will it cause aerosol contamination, but will also pollute the next samples.

2. Long Tips

Some tips are developed with a long shaft for specific lab applications. These were created with a common laboratory mistake in mind that can cause cross-contamination. Some technicians risk contamination by putting the shaft of a pipette attached to a standard tip into the tube to try to reach the bottom. This practice risks contamination, which can be eliminated by the use of pipette tips with an extended length. They are ideal for lab instruments such as deep well blocks and microcentrifuge tubes.

3. Short Tips

There are two major reasons for the development of short pipette tips. When you are manually pipetting into a multi-channeled, 1536 or 384 well plate, the small tips help to target the smaller wells. Additionally, they allow you to pipette near the bench which reduces the stress on the arm, resulting in improved ergonomics.

4. Low Retention Tips

These types of tips are designed to retain a lesser amount of liquid, leading to a more precise and consistent stream of results. However, the cost of these tips is higher than others, so it is recommended to use them strictly for specified applications.

5. Wide Bore Tips

A cellular sample should never be forced through the narrow opening of a standard tip. These samples are quite fragile and prone to shearing. Use wide bore tips when handling vulnerable cell samples, including cell lines and violent materials. When the sample is transferred through a wider orifice, it prevents resistance in the flow and shearing of the cellular components.

 

Application of Pipette Tips
 

Use the appropriate pipette tips
To ensure better accuracy and precision, it is recommended that the pipetting volume is in the range of 35% to 100% of the pipette tips range.

 

Installation of pipette tips
For most brands of pipettes, especially multichannel pipettes, installing the pipette tips is not an easy task. To have a good seal, the lower end of the pipette's sleeve needs to be inserted with a pipette tips and tightened by turning it left and right or rocking it back and forth. Some people may tight the tips by repeatedly hitting them into the pipettes, but this operation may lead to deformation of the tips and affect the accuracy, or even damage the pipette.

 

Angle and depth of pipette tipss immersion
The angle of pipette tips immersion should be in the tilt within 20 degrees and it is better to keep vertical.

 

Pipette Tips

Process of Pipette Tips

Material selection

The pipette tipss can be made from materials like polypropylene, polystyrene, or polyethylene. The choice of material depends on the application and the compatibility of the material with the sample.

 

Injection molding

The chosen material is melted and injected into molds to form the tip shape. The molds are made to precision to ensure that the tips are consistent in size and shape.

 

Cutting and polishing

After the injection molding process, the excess material is trimmed off, and the tips undergo a polishing process to ensure that the surfaces are smooth and free of any defects.

Quality control

Before the tips are packaged, they are tested for quality and consistency. The tips are checked for their accuracy, precision, and suitability for the intended application.

Packaging and labeling

The tips are packaged in boxes or racks, and each box or rack is labeled with product information like the volume, compatibility, and lot number.

Sterilization

To ensure that the tips are free of any contaminants, they are sterilized using methods like gamma irradiation or ethylene oxide sterilization.

Distribution

The pipette tipss are finally distributed to the end-users, who can use them for their intended applications.

 

 

What are the Parts/ Components of Pipette Tips

Pipette tipss are accessible in numerous designs and sizes. In any case, there are certain components that are basic and common to all the pipette tipss. These components of pipette tips are:

 

Plunger: It is mainly used to perform the two functions: Volume adjustment and Liquid aspiration/ dispensing

 

Tip Ejector: In order to allow the safe, effortless, and quick ejection of tips, pipette tipss are provided with a tip ejector.

 

Volume Display: This shows the volume of the liquid to be aspirated or dispensed.

 

Tipcone: The tipcone provides fitment to the tips. A pipette tips with a universal tipcone is recommended because it increases the instrument's compatibility with most of the standard tips.

 

Gripy: It helps to support and hold the micropipette comfortably.

Pipette Tips

 

How to Maintain Pipette Tips

 

Pipette tips are so commonly used in the laboratory that it can be easy to take them for granted. Without proper care and maintenance, pipette tipss can become less accurate and even cause sample contamination. The best way to prevent this source of error and frustration is to clean and maintain pipette tipss regularly.

 

●Daily
Clean the outside of the pipette tips with a lint-free cloth and 70 % ethanol. If a different decontamination solution is used, check that the pipette tips is chemically compatible with it. Perfoming this step daily will help to ensure a spotless workspace.

 

●Monthly
Perform a leak test and validate the pipetting volumes to make sure that your pipette tips is working as intended. If your pipette tips's accuracy and precision are not within specifications, it needs to be calibrated.

 

●Yearly
Step 1

Disassemble the pipette tips to clean each component. This can be performed by following the operating instructions for single channel pipette tipss, but multichannel pipette tipss should only be disassembled by trained personnel. To disassemble a single channel pipette tips, disconnect the upper and lower halves of the pipette tips and remove the O-ring and piston.

 

Step 2
Clean the pieces and remove any clogs in the airways. Check that there are no damaged parts and replace the O-rings of the tip fittings. Let the pipette tips air dry, then grease the piston with an appropriate pipette tips-safe lubricant, as recommended in the pipette tips's operating instructions. Reassemble the pipette tips and perform a leak test and validation of the pipetting volumes.

 

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Company Certificate
 

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FAQ
 

Q: What is a pipette tip used for?

A: Pipette Tips are disposable, autoclavable attachments for the uptake and dispensing of liquids using a pipette. Micropipettes are used in a number of laboratories. A research/diagnostic lab can use pipette tips to dispense liquids into a well plate for PCR assays.

Q: What is the function of tips in laboratory?

A: Tips function according to the positive-displacement principle, ensuring that the correct volume is dispensed with regard to the density and viscosity of the liquid used. The positive displacement system provides safer dispensing of radioactive and toxic substances as well as solutions with high vapor pressure.

Q: What is the difference between pipette tips and filter tips?

A: In summary, while both universal and filtered pipette tips serve the purpose of transferring liquids, filtered tips provide an additional layer of protection against contamination and are ideal for applications where sample purity is critical.

Q: What to do with used pipette tips?

A: EHS recommends collecting pipette tips intended for disposal as a chemical waste in a wide mouth jar (glass or plastic) with a screw capped lid. Large pipettes should be placed in a hard sided container, such as a box, with a plastic liner in case of leakage.

Q: What happens if you use a pipette without a tip?

A: Never use a micropipette without a tip. Always pipette gently, releasing the plunger button slowly. Letting the button pop up causes liquid to splash into the tip and can contaminate the plunger.

Q: How often should you change your pipette tip?

A: One box of pipette tips might look like a lot, but consider this: A pipette tip can only be used once. After you have used the pipette tip, it is contaminated by liquid you are pipetting. Usually you dispose of that tip and use a new pipette tip for the next sample.

Q: What is the purpose of filtered tips?

A: Filter tips serve two main purposes – they protect the pipette from the aerosol and protect the sample from the aerosol. Due to the functionality of the filtered tips, they are highly valuable for ultrasensitive applications like Polymerase Chain Reaction (PCR).

Q: Why do we always need a pipette tip?

A: Why do we always need a tip? You always need a pipette tip to reduce the risk of aerosol formation, protect the shafts from contamination, and minimize the risk of cross-contamination in the laboratory.

Q: Why is wiping the pipette tip necessary?

A: Clean Pipette Exteriors - Minimize pipette contamination and cross-contamination by wiping down the pipette each day, especially the tip end. Store Pipettes Vertically - Storing pipettes vertically minimizes pipette contamination, sample cross-contamination and damage.

Q: Why do you dispose of the pipette tips every time you use one?

A: Because PCR amplifies DNA, even the most minute amounts of DNA contamination can turn into big problems. In short, extremely small amounts of contaminating DNA can be disastrous. If a tip comes in contact with more than one reagent, throw it out.

Q: What are pipette tips for?

A: Filtered pipette tips are designed to prevent aerosols from forming. Aerosols are small liquid or solid particles that are airborne. These particles can actually remain airborne for long periods of time and can be inhaled. Even worse, 65% of all laboratory infections are caused by aerosols, usually by inhaling them.

Q: What is the difference between blue and yellow pipette tips?

A: Normally the 10ul pipette tip is clear, the 200ul pipette tip is yellow, the 1000ul pipette tip is blue. Disposable Plastic pipette tips fall into two category, one is DNAse RNAse free pipette tips and DEPC handled pipette tips.

Q: Can you cut pipette tips?

A: The pipette tips should be picked directly with the pipette from a pipette tip rack by gently pressing and ejected after use directly into a waste container. Never cut, bend or otherwise manipulate the pipette tips as it can affect your pipetting results.

Q: When can you use the same pipette tip?

A: In general, it is not recommended to reuse pipette tips between experiments. Pipette tips are designed to be disposable and single-use items for several reasons: Contamination: Reusing pipette tips increases the risk of cross-contamination between samples, which can lead to inaccurate and unreliable results.

Q: Why are pipette tips rarely reusable?

A: Sometimes, pipette tips could come into contact with other reagents. It does not matter if the reagents are harmful or corrosive, even though that is something to worry about in their own way. But no matter what the reagent in contact is, throw out the pipette tip right away.

Q: Why are pipette tips important?

A: If your experiment requires sterility, like cell culture, the use of pipette tips is crucial. They can not only reduce cross-contamination, but if you use the right kind of tips, you'll also get better results. Sterile tips are not often since not all scientific research needs to be sterile.

Q: Should you pre wet pipette tips?

A: Aspirate and fully expel an amount of the liquid at least three times before aspirating for delivery. Failure to pre-wet the tip increases evaporation within the tip air space, which can cause significantly lower delivery volumes. Pre-wetting increases the humidity within the tip, thus reducing evaporation.

Q: Can you recycle pipette tips?

A: Choosing tips made from recycled plastics or switching to glass may also be options. And there are pipette tip washers on the market for those who want to go the extra mile. Plastic pipette tips can absolutely be recycled, but not each waste hauler has the facilities or the confidence to do so.

Q: Can I reuse pipette tips?

A: Washing and reusing tips is a proven safe practice that has been validated by smart, proactive labs committed to turning single-use plastic into a multi-use resource to reduce waste, cut costs, and boost efficiency. The safe practice of washing and reusing disposable pipette tips will NOT damage lab equipment.

Q: What is the function of the tip of the pipette?

A: Pipette tips allow the accurate and reliable transfer of small volumes of liquid that is required in most areas of molecular biology. These micropipette tips are suitable for use with fixed and variable volume micropipettes, and are designed for accurate and precise liquid measurement.

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